Hoofprint Biome

High-Level Overview

Hoofprint Biome is a biotech startup founded in 2023 that develops probiotics and natural enzymes to engineer the cattle rumen microbiome, reducing methane emissions while improving animal health, feed efficiency, and productivity such as milk or beef yield[1][2][4][5]. It serves cattle farmers in dairy and beef operations by addressing enteric methane—responsible for about 6% of global greenhouse gas emissions and up to 12% of a cow's feed energy loss—through biology-based solutions rather than chemicals[1][3][4]. The company has raised approximately $19 million in funding, including a $4.25 million pre-seed round and a $15 million Series A in April 2025, with 11-50 employees and backing from investors like Breakthrough Energy Ventures, Amazon's Climate Pledge Fund, and SOSV's IndieBio accelerator[1][5][6].

Its first product is a yeast-enzyme blend (using probiotic Saccharomyces boulardii) designed for daily feed inclusion, which secretes lytic peptides and enzymes to eliminate methanogens, boost volatile fatty acids for better energy absorption, and increase profitability—demonstrated potential for over 5% gains in milk yield and feed efficiency[1][2][3].

Origin Story

Hoofprint Biome emerged from NC State University research, founded in 2023 by co-founders Kathryn Polkoff (CEO, Ph.D. from NC State's College of Veterinary Medicine in animal science) and Dr. Scott Collins (chemical engineer and board member, former researcher in NC State labs)[2][5]. The idea stemmed from insights into the cow rumen microbiome: methanogens waste up to 12% of feed energy, and Polkoff and Collins aimed to use biotechnology—like enzymes and AI—to boost yield and cut emissions simultaneously, licensing patented techniques from NC State professor John Crook[2][5].

Early traction came via IndieBio (SOSV), NC State's startup incubator, and SBIR grants totaling over $440,000 for probiotic yeast development, enabling rapid enzyme discovery with AI tools like AlphaFold—compressing a decade of work into under 12 months[1][2][3][5]. A pivotal $4.25 million pre-seed round in 2023 fueled conferences, investor pitches, and product prototyping[5].

Core Differentiators

• Biology-First Methane Reduction: Unlike chemical-based rivals (e.g., seaweed-derived bromoform), Hoofprint uses natural rumen enzymes, lytic peptides, and probiotic yeast to eliminate methanogens at the source without toxicity, while enhancing microbiome efficiency for higher volatile fatty acids, milk/beef output, and profitability[1][2][3][5].
• AI-Accelerated Discovery: Integrates machine learning and AlphaFold to screen thousands of enzymes from microbial "dark matter" DNA, enabling unprecedented speed in optimization[2].
• Dual Benefits Model: Solves farmers' economic barriers by improving performance (e.g., 5%+ feed efficiency/milk gains) alongside emissions cuts, making it scalable and cost-effective[1][3][4].
• Proven Delivery Platform: Engineered Saccharomyces boulardii probiotic secretes anti-methanogen agents directly in the rumen, with NC State-validated health perks[3][5].

Role in the Broader Tech Landscape

Hoofprint rides the climate-agritech wave, targeting livestock methane (nearly 10% of global GHG from rumen methanogens) amid net-zero food system mandates and dairy industry pushback on chemicals[1][3][4]. Timing aligns with AI-biotech convergence accelerating microbiome engineering, post-2023 funding surge in deep tech for sustainable protein, and market forces like carbon credits, regulations (e.g., EU methane pledges), and investor focus from Bill Gates' Breakthrough Energy[2][6].

It influences the ecosystem by pioneering "symbiotic agriculture"—mutually beneficial human-animal-planet systems—via upcycling cattle's grass/waste diet, potentially unlocking $billions in ag by proving biology trumps suppression tactics, and inspiring similar rumen tech for other emissions[4].

Quick Take & Future Outlook

Hoofprint's momentum—$19M raised, Series A in 2025, lab-to-feedlot product pipeline—positions it for commercialization by 2026, starting with yeast blends in U.S. dairy/feedlots, expanding globally via partners like AgriZeroNZ[1][2][6]. Trends like AI enzyme design, microbiome IP expansion, and symbiotic ag incentives will propel growth, evolving its influence from methane mitigator to cattle productivity leader amid scalability wins over chemical alternatives. This rumen revolution could redefine cattle as climate allies, tying back to its core promise: natural biology for profitable, planet-friendly farming[1][4].